Actinomycetes, 1992 Vol.3 Part 2 25-30
CLONING OF SINEFUNGIN BIOSYNTHETIC GENES FROM STREPTOMYCES
INCARNATUS TO STREPTOMYCES LIVIDANS
HALINA MALINA(1), FRANCOISE LAWRENCE, and MALKA ROBERT-GERO
Code Number: AC95006
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ABSTRACT. Biosynthetic genes (sin genes) of sinefungin, a
transmethylase inhibitor, antifungal and antiparasitic
nucleoside antibiotic produced by Streptomyces incarnatus,
were shotgun cloned into Streptomyces lividans. The genes are
clustered on a 14 kb fragment of DNA. The expression of the
sin gene cluster in S. lividans leads to the production of a
sinefungin-like antibiotic. The compound has antifungal and
antiparasitic activities in vitro. The expression of the gene
cluster in S.lividans and E. coli causes an astringent-like
response.
Copyright 1992 CETA
