Mouse UCP1, human UCP2 and UCP3 were expressed in yeast (
Saccharomyces cerevisiae
). Total phospholipids were extracted and their fatty acyl composition was analysed. Expression of hUCP2 and hUCP3 significantly altered the mitochondrial phospholipid fatty acyl composition. Palmitoleoyl groups (16:1(
n-7)) decreased while oleoyl groups (18:1(
n-9)) increased, leading to a significant increase in the 18:1(
n-9)/16:1(
n-7) ratio and in the average chain length. Expression of mUCP1 did not cause any significant changes in fatty acyl composition
