Pretreatment of the cellulosic substrate has miracle effect on the enhancement of cellulase production by fungal strains. A thermophilic strain of
Humicola insolens
TAS-13 was locally isolated and was tested for cellulases production under solid-state fermentation conditions using sugar cane bagasse as substrate. The cultural conditions for the
H. insolens were also optimized for the higher rate of cellulase secretion. In order to enhance the production rate of heterogenous cellulosic proteins, bagasse was pretreated with NaOH, H
2SO
4, H
2O
2 and H
2O
2+1.5%NaOH. The pretreatment of bagasse with 2.0% H
2O
2 along with 1.5% NaOH enhanced the biosynthesis of cellulases by
H. insolens. Production rate was also optimized with different parameters like thickness of fermentation medium, initial pH, incubation time and temperature. The thickness of the fermentation medium of 0.8 cm (10 g) with pH range of 5.5 was found to be better for enhanced production at 50°C. The yield of the enzyme was reached maximum with CMC-ase (18.98 U/g/min), FP-ase (13.63 U/g/min), β-glucosidase (19.54 U/g/min) 72 h after inoculation.
