Four isolates, isolated from fruit of sapodilla collected at Chantaburi and
designated as CT8-1 and CT8-2, and isolated from seeds of ixora („khem” in Thai,
Ixora species) collected at Rayong and designated as SI15-1 and SI15-2, were
examined taxonomically. The four isolates were selected from a total of 181 isolated
acetic acid bacteria. Isolates CT8-1 and CT8-2 were non motile and produced a
levan-like mucous polysaccharide from sucrose or D-fructose, but did not produce a
water-soluble brown pigment from D-glucose on CaCO
3-containing agar slants. The
isolates produced acetic acid from ethanol and oxidized acetate and lactate to
carbon dioxide and water, but the intensity of the acetate and lactate oxidation was
weak. Their growth was not inhibited by 0.35 % acetic acid (v/v) at pH 3.5. The
isolates did not grow on 30 % D-glucose (w/v), and utilization of methanol was not
found. Isolates SI15-1 and SI15-2 had peritrichous flagella and grew in the presence
of either 0.35 % acetic acid (v/v) at pH 3.5, 3 % NaCl (w/v), or 1 % KNO
3 (w/v).
Acetate and lactate were oxidized to carbon dioxide and water, but the intensity was
weak. The isolates grew on mannitol agar and glutamate agar as well as on 30 % Dglucose
(w/v), but did not utilize methanol. The 16S rRNA gene sequence analysis
and DNA-DNA hybridization indicated that isolates CT8-1 and CT8-2 and isolates
SI15-1 and SI15-2 were unequivocally identified respectively as
Kozakia baliensis
and
Swaminathania salitolerans
.
