In order to study the effects of organic (cattle manure) and bio-fertilizer (mycorrhiza) applications on physiological and
biochemical changes in soybean [
Glycine max
(L.) Merr.] grown under conditions of water stress, an experiment was
conducted at Varamin, Iran during 2011 growing season. The experimental design was laid out in a randomized complete
blocks with a split factorial arrangement of treatments in three replications. Main factor was water stress (normal irrigation
and irrigation withholding after 50% flowering) and sub factors were included cattle manure (0, 15 and 30 t per hectare) and
mycorrhiza application (with and without). The results showed that water stress significantly decreased relative water
content and electrolyte leakage while increased antioxidant enzyme activity, malondialdehyde content, dityrosine and
hydroxyguanosine. By contrast, mycorrhiza and cattle manure application had positive effect on relative water content and
improve of electrolyte leakage. These treatments alleviate enzyme activity and lipid or protein peroxidation. In general, it is
concluded that mycorrhiza and manure application can ameliorate growth conditions for soybean plants grown under water
deficit stress.
