The tomato leaf miner
Tuta absoluta
Meyrick has invaded tomato (
Solanum lycopersicum
L.) crop in Tunisia since 2008
and is representing today a major threat to the production of this crop. In this study, we used the Randomly Amplified
Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) technology to assess the genetic variability within and
among seven populations of
T. absoluta, collected on tomato from different regions in Tunisia. Using five RAPD-PCR
primers and 108 individuals, 140 polymorphic fragments were recorded. From 335 different RAPD phenotypes generated,
71 were redundant and 264 unique to a specific population. The genetic structure of
T. absoluta was investigated using
analysis of molecular variance (AMOVA), genetic distances (Fst) and multidimensional scaling (MDS). We detected a
high genetic diversity within and among populations in conjunction with a significant differentiation between populations,
suggesting that different founder genotypes would have been responsible of the introduction of
T. absoluta in Tunisia. The
presence of overlapping phenotypes probably indicates migration events between populations, mainly through infested
plant material carried by humans.
