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Chilean Journal of Agricultural Research
Instituto de Investigaciones Agropecuarias, INIA
ISSN: 0718-5820 EISSN: 0718-5820
Vol. 77, No. 3, 2017, pp. 257-265
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Bioline Code: cj17032
Full paper language: English
Document type: Research Article
Document available free of charge
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Chilean Journal of Agricultural Research, Vol. 77, No. 3, 2017, pp. 257-265
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Multiple resistance to ACCase and ALS-inhibiting herbicides in Beckmannia syzigachne (Steud.) Fernald without mutations in the target enzymes
Li, Wei; Zhang, Lele; Zhao, Ning; Guo, Wenlei; Liu, Weitang; Li, Lingxu & Wang, Jinxin
Abstract
American slough grass (Beckmannia syzigachne [Steud.]
Fernald) is a worldwide weed and is widely distributed
in rice-wheat rotations in China. Fenoxaprop-P-ethyl
and mesosulfuron-methyl are two major herbicides used
to control B. syzigachne. Resistance has evolved in B.
syzigachne under continuous selective pressure from
herbicides. This study aimed to establish the cross-resistance
pattern of a resistant population and explore the
potential non-target-site based resistance mechanisms of
B. syzigachne. Sequencing of target enzyme genes (acetyl
coenzyme A carboxylase [ACCase] and acetolactate
synthase [ALS]) revealed that there were no resistance-endowing
amino acid substitutions in the resistant B.
syzigachne population (RF1) compared with the sensitive
population (SF1), and obtained the purified materials.
Furthermore, piperonyl butoxide (PBO) and malathion
showed synergistic effects with fenoxaprop-P-ethyl and
mesosulfuron-methyl respectively in RF1. Therefore, we
speculate that the resistance observed in B. syzigachne was
related with metabolic, mostly involving the cytochrome
P450 enzymes. Cross resistance patterns showed that the
purified resistant B. syzigachne produce high resistance
to fenoxaprop-P-ethyl and pyroxsulam; intermediate
resistance to flucarbazone-sodium; low resistance to
quizalofop-P-ethyl, clodinafop-propargyl, sethoxydim and
mesosulfuron-methyl; sensitive to clethodim pinoxaden
and isoproturon.
Keywords
Herbicide resistance; metabolic resistance; P450 inhibitors; target enzymes sequencing.
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