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African Crop Science Journal
African Crop Science Society
ISSN: 1021-9730 EISSN: 1021-9730
Vol. 5, No. 2, 1997, pp. 197-200
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Bioline Code: cs97025
Full paper language: English
Document type: Short Communication
Document available free of charge
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African Crop Science Journal, Vol. 5, No. 2, 1997, pp. 197-200
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Umoh,E. O.
Résumé
D'une facon generale, on obtient les protoplastes de Sorghum bicolor
Moench a partir des cellules en suspension et purifiees a l'aide des
methodes de sedimentation ou de flottaison de sucrose. Les effets de ces
deux methodes ont ete evalues en utilisant les protoplastes de la tige
etiolee de deux cultivars de sorgho. Les taux de recuperation se sont
averes plus eleves dans la methode de sedimentation que dans celle de
flottaison du sucrose. La methode de sedimentation n'a pas montre un
changement significatif des rapports protoplastes vacuoles sur protoplastes
etioles dans les echantillons initiaux et purifies. Par ailleurs, ces
proportions ont ete en grande partie titrees en faveur du protoplaste
vacuole par la methode de sedimentation du sucrose. On pourrait ainsi
exploiter a resultat et selectionner la methode de purification appropriee
en se basant sur les besoins experimentaux specifiques.
Mots Clés
Tiges etiolees, purification des protoplastes, sorgho
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en |
A comparative assessment of the purification techniques for etiolated seedling protoplast of Sorghum bicolor
Umoh,E. O.
Abstract
Protoplasts of Sorghum bicolor Moench are usually obtained from
suspension cells and purified using either the sedimentation or the sucrose
floatation methods. Effects of these two methods were assessed using
etiolated shoot protoplats of two cultivars of sorghum. Recovery rates
were higher in the sedimentation method than in the sucrose floatation
method. The ratios of vacuolated to etioplastic in the initial and
purified samples of protoplasts were not markedly changed with the
sedimentation method. On the other hand, these ratios were largely titled
in favour of vacuolated protoplast when the sucrose floatation method was
used. This finding could be exploited and an appriopriate purification
method selected based on the specific experimental requirements.
Keywords
Etiolated shoots, protoplast purification, sorghum
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© Copyright 1997 - African Crop Science Society
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