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African Crop Science Journal
African Crop Science Society
ISSN: 1021-9730 EISSN: 1021-9730
Vol. 6, No. 1, 1998, pp. 69-78
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Bioline Code: cs98008
Full paper language: English
Document type: Research Article
Document available free of charge
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African Crop Science Journal, Vol. 6, No. 1, 1998, pp. 69-78
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Occurrence of Telfairia mosaic virus (temv) in fluted pumpkin in Nigeria
Shoyinka, S.A. & Thottappilly, G.
Abstract
Fluted pumpkin (Telfaira occidentalis, Hook F.) fields, experimental
plots and private gardens in 12 major pumpkin producing states in southern
Nigeria were surveyed between 1987 and 1989 for relative incidences,
distribution and prevalence of three viruses, namely, Telfairia
mosaic potyvirus (TeMV), pepper veinal mottle potyvirus (PVMV) and Y-strain
of cucumber mosaic cucumovirus (CMV). Using electron microscopy, host-plant
reactions and enzyme-linked immunosorbent assay (ELISA) either alone or in
combination, a total of 584 leaf samples from 85 sites was tested for the
presence of the three viruses reported to infect the crop. Out of a total
of 584 samples tested serologically, 498 (representing 85%) reacted
positively to TeMV. However, no positive reaction was obtained when samples
were tested against antisera to CMV or PVMV. TeMV infection was widely
distributed in the area surveyed and its incidence varied considerably
reaching 100% in some areas. Neither PVMV nor CMV infected fluted pumpkin
in greenhouse tests but the two viruses were detected in their respective
plant-hosts, pepper and cowpeas, growing with fluted pumpkin in the same or
adjacent locations. No evidence of mixed infections with the three viruses
was obtained from studies by host range, electron microscopy, or
serological assay. The high incidences and wide distribution of TeMV
recorded in this survey indicates that the virus may be of economic
importance in the production of this vegetable in Nigeria.
Keywords
Cucumber mosaic cucumovirus, electron microscopy, greenhouse bioassay, pepper veinal mottle potyvirus, serological detection, Telfairia mosaic potyvirus
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fr |
Shoyinka, S.A. & Thottappilly, G.
Résumé
Les champs de la citrouille (potrion) a cannelures, les parcelles
experimentales et les jardins privees dans 12 etats producteurs de la
citrouille au sud du Nigeria ont ete etudiees entre 1987 et 1989 en ce qui
concerne les incidences relatives, la distribution et la prevalance des
trois virus, notamment le potyvirus de la mosaique Telfairia (TeMV),
le potyvirus de la teche verinee du poivre et la race - Y de la mosaique
cucumovirus du concombre. Avec l'aide du microscope electronique, les
reactions de la plante hote et l'essai de l'enzyme immunosorbante (ELISA)
seul ou en combinaison, un total de 584 echantillons de feuilles provenant
de 85 sites a ete teste pour evaluer la presence des trois virus
susceptibles d'infecter la culture. Sur un total de 584 echantillons testes
serologiquement, 498 (soit 85%) ont reagi positivement au TeMV. Toutefois,
aucune reaction n'a ete obtenue lorsque les echantillons etaient testes
contre l'antisera au CMV ou au PVMV. L'infestion TeMV a ete largement
repandue sur la surface etudiee et son incidence variait considerablement
jusqu' a 100% sur certaines surfaces. Ni PVMV ni CMV n'ont infecte la
citrouille a cannelures dans les tests en serre mais les deux virus ont ete
detectes dans leurs plantes-hotes respectives, le poivre et le cerfeuil,
croissant ensemble avec la citrouille a cannelures aux memes endroits ou
presque. Aucune evidence des infections mixtes avec les trois virus n'a ete
obtenue des etudes sur ou par de nombreux hotes, a la aide d'un microscope
electronique ou d'un essai serologique. Les grandes incidences et la large
distribution de TeMV enregistres dans cette etude indiquent que le virus
peut avoir une grande importance economique dans la production de ce legume
au Nigeria.
Mots Clés
Mosaique cucumovirus du concombre, microscope electronique, Telfairia occidentalis, Hook F., serre, potyvirus de la teche veinee du poivre, detection serologique, mosaique potyvirus de Telfairia
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© Copyright 1998 - African Crop Science Society
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