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A laboratory scale device for microencapsulation of genetically engineered cells into alginate beads
Fiszman, Gabriel León; Karara, Armando Luis; Finocchiaro, Liliana María Elena & Glikin, Gerardo Claudio
Abstract
The microencapsulation of recombinant cells, widely used for in vitro high-density cell culture, is a novel and potentially cost-effective method of in vivo heterologous protein delivery, where the protein producing cells are immunologically protected from tissue rejection. We report here a simple, reliable and inexpensive laboratory method to generate calcium alginate microcapsules containing genetically engineered, interleukin-2 expressing, Chinese hamster ovary (CHO) cells.
Keywords
CHO cells, interleukin-2, protein delivery
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