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Ethanol mediated enhancement in bacterial transformation
Sharma, Arun Dev; Singh, Jaspreet & Gill, Prabhjot Kaur
Abstract
In molecular biology, transformation using E. coli as a host plays a key role in synthesizing gene libraries. The present study demonstrated a new ethanol-based method for transformation of plasmid DNA to E. coli. Ethanol at 10% concentration (v/v) showed best results. Further, as compared with traditional CaCl2 method, the transformation rate, using protocol outlined in this study, was very high, suggesting amenable for further applications.
Keywords
bacteria, ethanol, transformation.
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