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Genetic diversity of Brassica oleracea var. capitata gene bank accessions assessed by AFLP
Faltusová, Zuzana; Kučera, Ladislav & Ovesná, Jaroslava
Abstract
The genetic diversity of
20 cabbage (Brassica oleracea var. capitata, including sub.var. alba and rubra) cultivars and landraces from the Gene bank of Crop Research
Institute was estimated using amplified fragment length polymorphism (AFLP)
marker technology. Two cultivars of Brassica pekinensis (syn. Brassica
rapa var. pekinensis) were used as outliers in the study. Thirty
AFLP primer combinations produced a total of 1084 fragments. A total of 806
fragments, 364 (45%) of them polymorphic, were found across 20 Brassica
oleracea var. capitata accessions. The accessions were clustered
into two main groups. Special subgroups, reflecting place of origin, were
observed within these groups. Ten selective primer pairs were found to be most
informative because each of these uniquely identified all of the accessions
used. Furthermore, two accessions of Brassica pekinensis were clearly
differentiated from the Brassica oleracea var. capitata accessions. AFLP is an efficient tool for determination of genetic diversity of
cabbage gene bank accessions.
Keywords
amplified fragment length polymorphism, Brassica oleracea var. capitata, cabbage, discriminatory power, genetic diversity
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