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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 20, No. 1, 2016, pp. 28-32
Bioline Code: ej16015
Full paper language: English
Document type: Short Communication
Document available free of charge

Electronic Journal of Biotechnology, Vol. 20, No. 1, 2016, pp. 28-32

 en Chilean IPNV isolates: Robustness analysis of PCR detection
Jorquera, Esteban; Morales, Paz; Tapia, David; Torres, Pamela; Eissler, Yoanna; Espinoza, Juan C.; Conejeros, Pablo & Kuznar, Juan

Abstract

Background: The genomes of several infectious pancreatic necrosis viruses (IPNVs) isolated in Chile were sequenced with a single amplification approach for both segments A and B. The resulting sequences were then used to determine the conservation of the primer-binding regions used in polymerase chain reaction (PCR)-based diagnostic methods proposed in the literature. Thus, the robustness of each technique was studied, particularly the eventual effect of further mutations within the primer-binding sites.
Results: On analysis, most methods currently used to detect Chilean IPNV varieties were deemed adequate. However, the primers were designed to be genogroup specific, implying that most detection methods pose some risk of detecting all strains prevalent in the country, due to the coexistence of genogroups 1 and 5.
Conclusions: Negative resultsmust be interpreted carefully given the high genomic variability of IPNVs. Detection techniques (quantitative reverse transcription (qRT)-PCR) based on degenerate primers can be used to minimize the possibilities of false-negative detections.

Keywords
IPNV detection; Mismatch\'s Tm analysis; QPCR

 
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