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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 22, No. 1, 2016, pp. 16-25
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Bioline Code: ej16031
Full paper language: English
Document type: Research Article
Document available free of charge
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Electronic Journal of Biotechnology, Vol. 22, No. 1, 2016, pp. 16-25
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Statistical optimization of thermo-alkali stable xylanase production from Bacillus tequilensis strain ARMATI
Khusro, Ameer; Kaliyan, Barathi Kannan; Al-Dhabi, Naif Abdullah; Arasu, Mariadhas Valan & Agastian, Paul
Abstract
Background: Xylanase from bacteria finds use in prebleaching process and bioconversion of lignocelluloses into
feedstocks. The xylanolytic enzyme brings about the hydrolysis of complex biomolecules into simple monomer
units. This study aims to optimize the cellulase-free xylanase production and cell biomass of Bacillus tequilensis
strain ARMATI using response surface methodology (RSM).
Results: Statistical screening ofmediumconstituents and the physical factors affecting xylanase and biomass yield
of the isolate were optimized by RSM using central composite design at N = 30, namely 30 experimental runs
with 4 independent variables. The central composite design showed 3.7 fold and 1.5 fold increased xylanase
production and biomass yield of the isolate respectively compared to ‘one factor at a time approach’, in the
presence of the basal medium containing birchwood xylan (1.5% w/v) and yeast extract (1% w/v), incubated at
40°C for 24 h. Analysis of variance (ANOVA) revealed high coefficient of determination (R2) of 0.9978 and
0.9906 for the respective responses at significant level (p < 0.05). The crude xylanase obtained from the isolate
showed stability at high temperature (60°C) and alkaline condition (pH 9) up to 4 h of incubation.
Conclusions: The cellulase-free xylanase showed an alkali-tolerant and thermo-stable property with potentially
applicable nature at industrial scale. This statistical approach established a major contribution in enzyme
production from the isolate by optimizing independent factors and represents a first reference on the
enhanced production of thermo-alkali stable cellulase-free xylanase from B. tequilensis.
Keywords
Bacillus tequilensis; Biomass; Central composite design; RSM; Xylanase
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