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In vitro propagation of Vitis vinifera L. cv. ‘Monastrell’
Pedro, Tània San; Peiró, Rosa; Villanova, Joan; Olmos, Antonio & Gisbert, Carmina
Abstract
Background: A protocol for the micropropagation of the grape (Vitis vinifera L.) cultivar ‘Monastrell’ was
developed. Initial plant material was obtained from the sanitary selection of grapevine plants performed by
real-time RT-PCR to confirm the absence of Grapevine fanleaf virus, Arabis mosaic virus, Grapevine
leafroll-associated virus 1, Grapevine leafroll-associated virus 3, and Grapevine fleck virus.
Results: The effects of the salt composition (comparing Lloyd and McCown woody plant medium and Murashige
and Skoog medium 1/2 macronutrients) and the growth regulator benzylaminopurine (BAP), at 0 and 8.9 μM, on
plant propagation were evaluated using nodes as explants. The most efficient procedure consisted of bud
induction in the medium with Lloyd and McCown woody plant salts and 8.9 μM BAP for 30 d along with
elongation in cytokinin-free medium for 60 d, which gave 22 nodes/explant (174 plants/initial plant). A
second cycle of propagation in a medium without BAP for another 60 d could give approximately 10,000
nodes, which can be obtained after an additional 2 months of culture. All plants acclimatized after the second
cycle of multiplication were successfully transferred to soil.
Conclusion: We developed an optimal protocol for V. vinifera cv. ‘Monastrell’ micropropagation, the first described
for this cultivar.
Keywords
Mourvedre; Bud induction; Grapevine; Micropropagation; Mineral salts; Node explants; Quality wines; Real-time RT-PCR; Virus-free grapevine; Vitis vinifera micropropagation; Wineries
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