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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 28, No. 1, 2017, pp. 113-119
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Bioline Code: ej17055
Full paper language: English
Document type: Research Article
Document available free of charge
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Electronic Journal of Biotechnology, Vol. 28, No. 1, 2017, pp. 113-119
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Molecular characterization of CHST11 and its potential role in nacre formation in pearl oyster Pinctada fucata martensii
Wang, Qingheng; Yang, Chuangye; Hao, Ruijuan; Zheng, Zhe; Jiao, Yu; Du, Xiaodong; Deng, Yuewen & Huang, Ronglian
Abstract
Background: C4ST-1 catalyzes the transfer of sulfate groups in the sulfonation of chondroitin during chondroitin
sulfate synthesis. Chondroitin sulfate consists of numerous copies of negatively charged sulfonic acid groups that
participate in the nucleation process of biomineralization. In the present study, we obtained two CHST11 genes
(PmCHST11a and PmCHST11b) which encoded the C4ST-1 and explored the functions of these genes in the
synthesis of chondroitin sulfate and in the formation of the nacreous layer of shells.
Results: Both PmCHST11a and PmCHST11b had a sulfotransferase-2 domain, a signal peptide and a
transmembrane domain. These properties indicated that these genes localize in the Golgi apparatus. Real-time
PCR revealed that both PmCHST11a and PmCHST11b were highly expressed in the central zone of the mantle
tissue. Inhibiting PmCHST11a and PmCHST11b via RNA interference significantly decreased the expression
levels of these genes in the central zone of the mantle tissue and the concentration of chondroitin sulfate in
extrapallial fluid. Moreover, shell nacre crystallized irregularly with a rough surface after RNA interference.
Conclusions: This study indicated thatPmCHST11a and PmCHST11b are involved in the nacre formation of Pinctada
fucata martensii through participating in the synthesis of chondroitin sulfate.
Keywords
Biomineral; Biomineralization; Carbohydrate sulfotransferase 11; Chondroitin sulfate; Chondroitin-4-sulfotransferase-1 (C4ST-1); Mantle tissue; Molluscs; Proteoglycans; Real-time PCR; RNA interference
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