Background: CDC25 is a dual-specificity phosphatase that was first identified in the yeast
Schizosaccharomyces pombe
as a cell cycle-defective mutant. Although CDC25 is involved in the cell cycle of ovarian granulosa cells,
the CDC25 signaling pathway has not been clarified fully. To explore the role of
CDC25C in the cell cycle of goat
ovarian granulosa cells, a
CDC25C-overexpressing vector, pCMV-HA-CDC25C, was constructed and transfected
into granulosa cells from adult and young white goats from Jiangsu Nantong. RT-PCR was used to measure
CDC25C,
CDK1, and
WEE1 gene expression levels, and flow cytometry was used to distinguish ovarian
granulosa cells in different phases of the cell cycle. Progesterone and estradiol levels in transfected ovarian
granulosa cells were also measured.
Results: In adult goat follicular granulosa cells transfected with pCMV-HA-CDC25C, CDC25C expression increased
significantly, which greatly increased the relative gene expression levels of both CDK1 and WEE1. Additionally,
progesterone and estradiol levels were increased in goat follicular granulosa cells overexpressing CDC25C.
And the cell cycle results showed that transfection of pCMV-HA-CDC25C leads to a higher proportion of cells
in S phase compared to the no vector-transfected groups.
Conclusions: The results of this study indicated that the overexpression of
CDC25C may increase the gene
expression levels of both
WEE1 and
CDK1 in S phase and accelerate the transition of cells from G1 phase to
S phase.
