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Role of Peroxisome Proliferator- Activated Receptor gamma2 (PPAR-γg2) Gene Polymorphism in Type 2 Diabetes Mellitus
Sokkar, Salwa; El-Sharnouby, Jehan A.; Helmy, Amal; El-Bendary, Amal; Ahmad, Laila Sayed & Okasha, Kamal
Abstract
Objective:
To find out the relation between Pro 12 Ala polymorphism
of peroxisome proliferator- activated receptor gamma2
(PPAR-g2) gene with type 2 diabetes mellitus and the possible role
of this gene polymorphism as a link between obesity and type 2
diabetes mellitus.
Subjects and Methods:
Subjects of this study were classified
into 3 groups: (15) Apparently healthy lean individuals (Group I),
(15) obese non diabetic individuals (group II) and (24) patients
with type 2 diabetes mellitus (group III). This group divided into:
Diabetic non obese patients (12 patients) (Group IIIa) and: Diabetic
obese patients (12 patients) (Group IIIb). The subjects were subjected
to clinical examination, serum insulin level and estimation
of PPAR-g2 gene polymorphism by Restriction fragment length polymorphism
(RFLP) polymerase chain reaction (PCR).
Results:
Frequency of Pro allele was significant increase in diabetic
non obese patients& diabetic non obese patients when compared
to control group (P= 0.048 and 0.003, respectively). However, there
was no difference between obese non diabetic group and control
group as regard the allelic frequencies (p= 0.462). While diabetic
non obese patients had higher Pro allele frequency (p= 0.003) than
in diabetic obese patients (p= 0.048). Insulin resistance by homeostasis
model assessment (HOMA-IR) was significantly increased in
Pro/Pro carriers in group II and group III when compared to that
of Pro/Ala plus Ala/Ala carriers (p= 0.004 and 0.023; respectively).
Conclusion:
Pro12 allele of PPAR-g2 gene could be a genetic risk
factor for insulin resistance and type 2 diabetes mellitus.
Keywords
Diabetes mellitus, PPAR-g2 gene, obesity, insulin resistance
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