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African Health Sciences
Makerere University Medical School
ISSN: 1680-6905
EISSN: 1680-6905
Vol. 9, No. 1, 2009, pp. 34-39
Bioline Code: hs09006
Full paper language: English
Document type: Research Article
Document available free of charge

African Health Sciences, Vol. 9, No. 1, 2009, pp. 34-39

 en The in vitro antimicrobial activity of fruit and leaf crude extracts of Momordica charantia check for this species in other resources : A Tanzania medicinal plant
Mwambete, K. D.

Abstract

Objective: To evaluate the antimicrobial activity of Momordica charantia check for this species in other resources extracts on reference strains and microorganisms isolated from clinical specimens.
Method: Petroleum ether and methanolic crude extracts of fruits and leaves of the plant were evaluated for antimicrobial activity using the disk diffusion method on four reference microorganisms ( Pseudomonas aeruginosa check for this species in other resources , Escherichia coli check for this species in other resources , Candida albicans check for this species in other resources and Staphylococcus aureus check for this species in other resources ; and four clinical strains of Klebsiella pneumoniae check for this species in other resources , Proteus vulgaris check for this species in other resources , Salmonella typhi check for this species in other resources and Cryptococcus neoformans check for this species in other resources .
Result: Antimicrobial activity was observed against all the tested microorganisms with exception to P. mirabilis and C. neoformans. Methanolic crude extracts exhibited relatively broader antimicrobial spectrum of activity than petroleum ether extracts with the as lower concentration as 0.075mg/µl. Methanolic fruit crude extract displayed the broadest antimicrobial spectrum by inhibiting majority (75%) of the tested microorganisms. Neither was there synergistic nor addition effect upon mixing leaf and fruit extracts of equal concentrations derived from the same solvent.
Conclusion: Extracts of M.charantia demonstrated antimicrobial activity on tested microorganisms except on Proteus mirabilis check for this species in other resources and Cryptococcus neoformans. Fruit extracts showed higher antimicrobial activity than leaf extract. Further studies are recommended that will involve various parts of the plant, select different fractions of extracts and purify the active antimicrobial components.

Keywords
Antimicrobial activity, Momordica charantia, petroleum ether and methanolic crude extracts

 
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