The reconstitution of cellular immune components contributes to clinical outcome of HIV and Mycobacterium tuberculosis
(MTB) infection. Interruption of anti-retroviral therapy (ART) could lead to perturbations in reconstitution of T cells
in HIV/ tuberculosis (TB) patients.
To ascertain the effect of interrupted ART on reconstitution of CD4+
T sub-sets in TB patients.
Participants with HIV (CD4>350 cells/µL) and TB were recruited under a larger phase 3 open label randomised
controlled clinical trial. The CD45RO and CD62L markers were measured on CD4+
cells by flow cytometry. Samples
were analysed at baseline, 3, 6, 12 months.
There was a significant increase of naive CD8+
cells (p = 0.003) and a decrease in effector CD8+
cells (p = 0.004)
among participants in ART/TB treatment arm during the first 6 months. Withdrawing ART led to naive CD8+
(p=0.02) to values close to baseline. An increase of naive CD8+
cells after 6 months of TB treatment in TB alone treatment arm
(p=0.01) was observed. A trend towards increment of naive CD4+
sub sets in either treatment arms was observed.
Interrupting ART alters CD8+
but not CD4+
sub-sets in patients with less advanced HIV infection and TB.