have increasingly been associated with infections worldwide and anti-microbial resistance has
made these versatile pathogens more recalcitrant in the hospital setting.
This study sought to investigate the occurrence and distribution of Staphylococcus
species as well as determine the
prevalence of methicillin resistant Staphylococcus aureus
(MRSA) and methicillin resistant coagulase negative staphylococci
among clinical samples from University of Benin Teaching Hospital (UBTH) in Benin City.
Ninety one (91) clinical isolates comprising S. aureus
and Coagulase Negative staphylococci
(CoNS) were recovered from
routine clinical specimens and anti-microbial susceptibility tests were carried out. Polymerase Chain Reaction (PCR) was thereafter
carried out on these isolates to detect mecA
species had its highest prevalence from infected wounds of patients (28.8%) while urine samples showed
the least (5.4%). The highest level of resistance was to ceftazidime (S. aureus
- 68%, CoNS - 75.6%) while the least resistance
was observed for meropenem (S. aureus
- 26%, CoNS- 46.3%). Using phenotypic method (with 1µg oxacillin antibiotic disc), the
distribution of MRSA and MRCoNS was 44.0% and 46.3% respectively. PCR analysis showed that 38.0% of S. aureus
of the CoNS had mecA
gene respectively; wound swabs showed the highest prevalence with 30.5% of staphylococcal
mecA gene positive. There was also no significant association between the Staphylococcal
isolates and their isolation rate, isolation
site and mecA
gene distribution (p > 0.05).
This study draws attention on the increase in the prevalence of mecA
gene (39.6%) and an increase in multidrug
when compared to previous studies in our country; it recommends laboratory guidance and periodic review
to stem the tide of resistance.