Extract from the sea anemone,
Bunodosoma cavernata
was prepared by homogenising 100 of the sea anemones in 300ml of 0.9% saline in a Moulinex blender. This was centrifuged at 4000 revolutions per minute for 10 minutes. The supernatant was re - centrifuged to obtain the crude extract. The extract (1ml/l) was found to elicit long lasting contractions on the ilea of wild rabbit and grass cutter. It was also found to block acetylcholine (ACh) - induced contractions of the ileum of grass cutter (90 ± 6.2% inhibition ± SEM) and the contractile action induced by ACh (0.2μg and 0.4μg) (89 ± 5.8% inhibition ± SEM) and histamine (0.075μg and 0.15μg) on the rabbit ileum (80 ± 4.6% inhibition ± SEM). The antagonism by the extract was non - selective.
@JASEM
