The regeneration of transgenic plants of Eucalypt is the largest difficulty for the genetic transformation of this
genus; in addition a low rate of transformed plants is reached. The aim of this research was to evaluate
acetosyringone (3',5'-Dimethoxy-4'-hydroxyacetophenone) on the co-culture medium during genetic transformation
of shoot tips of
Eucalyptus saligna
via
Agrobacterium tumefaciens
and to promote the explants selection supposedly
transformed. Shoot tip from multiple shoots was used as explants. These explants were pre-cultured during two days
before the transformation. Strain EHA105 of
A. tumefaciens harboring the plasmid pBI121 was used. The
treatments were: 0 and 100 μM acetosyringone added to the co-culture, after co-culture the explants were cultured
in multiplication medium supplemented with 250 mg.L
-1 Cefotaxime® and each sub-culture the kanamycin levels
were increased from 50 to 150 mg.L
-1. The transient expression of the uidA gene in shoot tips was evaluated after
the end of the co-culture (fifth day) and after seven days of culture on medium containing kanamycin. The presence
of 100 µM acetosyringone at the co-culture of shoot tips of
Eucalyptus saligna promoted higher transient expression
of the uidA gene and retards toxic effects caused by kanamycin.