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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 95, No. 2, 2000, pp. 221-223
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Bioline Code: oc00037
Full paper language: English
Document type: Research Article
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 95, No. 2, 2000, pp. 221-223
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Short Communication: A Simple Method for Human Peripheral Blood Monocyte Isolation
Marcos C de Almeida; Alan C Silva; Aldina Barral & Manoel Barral Netto
Abstract
We describe a simple method using percoll gradient for isolation of highly enriched human monocytes. High numbers of fully functional cells are obtained from whole blood or buffy coat cells. The use of simple laboratory equipment and a relatively cheap reagent makes the described method a convenient approach to obtaining human monocytes.
{P}Key words:
Keywords
monocytes - percoll - gradient-isolation
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© Copyright 2000 Fundacao Oswaldo Cruz - Fiocruz
Alternative site location: http://memorias.ioc.fiocruz.br
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