|
Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 96, No. 6, 2001, pp. 835-838
|
Bioline Code: oc01135
Full paper language: English
Document type: Research Article
Document available free of charge
|
|
Memórias do Instituto Oswaldo Cruz, Vol. 96, No. 6, 2001, pp. 835-838
en |
SHORT COMMUNICATION - Identification of a Differentially Expressed mRNA in Axenic Leishmania panamensis Amastigotes
José Arturo Gutiérrez; Fabiola Puentes; Alberto Moreno; Manuel Elkin Patarroyo & Luis Angel Murillo
Abstract
Differential display technique was applied in order to identify transcripts which are present in axenic
amastigotes but not in promastigotes of the Leishmania panamensis parasites. One of them was cloned and
the sequence reveals an open reading frame of 364 amino acids (aprox. 40 kDa). The deduced protein is
homologous to the serine/threonine protein kinases and specially to the mitogen activates protein kinases
from eukaryotic species. Southern blot analysis suggest that this transcript, named lpmkh, is present in the genome of the parasite as a single copy gene. These results could imply that lpmkh could be involved in the differentiation process or the preservation of amastigotes in axenic conditions.
Keywords
differential display, axenic amastigotes, Leishmania panamensis, mitogen activated protein kinase
|
|
© Copyright 2001 Mem Inst Oswaldo Cruz Alternative site location: http://memorias.ioc.fiocruz.br
|
|