In a prospective field study conducted from July 2000 to June 2001, adult
Aedes aegypti
and
Ae.
albopictus
mosquitoes were caught from the municipality of Nova Iguaçu, State
of Rio de Janeiro, Brazil. Virus isolation in Ae. albopictus clone C6/36
cell line and a semi-nested reverse transcription-polymerase chain reaction
detected only dengue virus type 3 in three pools of
Ae. aegypti
, despite the co-circulation of DEN-1, DEN-2 and DEN-3 serotypes in that
area. No viruses were detected in
Ae. albopictus
mosquitoes. This virological surveillance consists in a sentinel system
alerting for dengue outbreaks.