An isoenzymatic comparative analysis of the variability and genetics differentiation
among
Anopheles
species was done in populations of
An. (Nys.) intermedius
and
An. (Ano.) mattogrossensis
of the
Anopheles
subgenus, and of
An. darlingi
,
An. albitarsis
and
An. triannulatus
of the
Nyssorhynchus
subgenus, with the aim of detecting differences between both subgenera and of
estimating the degree of genetic intere specific divergence. Samples from Macap
á
, State of Amap
á
and Janauari Lake, near Manaus, State of Amazonas, were analyzed for eight isoenzymatic
loci. Analysis revealed differences in the average number of alleles per locus
(1.6-2.3) and heterozygosity (0.060-0.284). However, the proportion of polymorphic
loci was the same for
An. (Nys.) darlingi
,
An. (Nys.) triannulatus
and
An. (Ano.) mattogrossensis
(50%), but differed for
An. (Nys.) albitarsis
(62.5%) and
An. (Ano.) intermedius
(25%). Only the IDH1 (P
>
0.5) locus in all species studied was in Hardy-Weinberg equilibrium. The fixation
index demonstrated elevated genetic structuring among species, based on values
of Fst = 0.644 and genetic distance (0.344-0.989). Genetic difference was higher
between
An. (Nys.) triannulatus
and
An. (Ano.) intermedius
(0.989) and smaller between
An. (Nys.) albitarsis
sensu lato and
An. (Nys.) darlingi
(0.344). The data show interspecific genetic divergence which differs from the
phylogenetic hypothesis based on morphological characters.
