We show for the first time that the ventral diverticulum of the mosquito gut (impermeable sugar storage organ) harbors microorganisms. The gut diverticulum from newly emerged and non-fed
Aedes aegypti
was dissected under aseptic conditions, homogenized and plated on BHI medium. Microbial isolates were identified by sequencing of 16S rDNA for bacteria and 28S rDNA for yeast. A direct DNA extraction from
Ae. aegypti gut diverticulum was also performed. The bacterial isolates were:
Bacillus sp.,
Bacillus subtilis
and
Serratia sp. The latter was the predominant bacteria found in our isolations. The yeast species identified was
Pichia caribbica
.
