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Comparison of HPV genotyping by type-specific pc r and sequencing
Carvalho, Nara de Oliveira; del Castillo, Dora Méndez; Perone, Carlos; Januário, José Nélio; de Melo, Victor Hugo & Filho, Geraldo Brasileiro
Abstract
Human papillomavirus (HPV) infection is the most common sexually transmitted disease worldwide and there
is a strong link between certain high-risk viral types and cervical carcinogenesis. Although there are several typing
methods, it is still unclear which test is the best. This study compared the effectiveness of type-specific PCR (TSPCR)
and sequencing, with a focus on their clinical application. A total of 260 cervical samples from HPV-positive
patients were tested for types 6, 11, 16, 18, 31, 33 and 35 using TS-PCR and sequencing. The genotype was identified
in 36% of cases by TS-PCR and in 75% by sequencing. Sequencing was four times more likely to identify the viral
type in positive samples than TS-PCR (p = 0.00). Despite being more effective for virus genotyping, sequencing was
unable to identify viral types in multiple infections. Combining both techniques resulted in highly sensitive detection
(87% of cases), showing that they are complementary methods. HPV genotyping is an important step in HPV
management, helping to identify patients with a higher risk of developing cervical cancer and contributing to the
development of type-specific vaccines.
Keywords
HPV - PCR - sequencing - typing
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