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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 105, No. 1, 2010, pp. 92-98
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Bioline Code: oc10014
Full paper language: English
Document type: Research Article
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 105, No. 1, 2010, pp. 92-98
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Sequencing of E2 and NS5A regions of HCV genotype 3a in Brazilian patients with chronic hepatitis
Malta, Fernanda de Mello; de Medeiros-Filho, José Eymard Moraes; de Azevedo, Raymundo Soares; Gonçalves, Luzia; da Silva, Luiz Caetano; Carrilho, Flair José & Pinho, João Renato Rebello
Abstract
Hepatitis C virus (HCV) is a major cause of liver disease throughout the world. The NS5A and E2 proteins of
HCV genotype 1 were reported to inhibit the double-stranded (ds) RNA-dependent protein kinase (PKR), which is
involved in the cellular antiviral response induced by interferon (IFN). The response to IFN therapy is quite different
between genotypes, with response rates among patients infected with types 2 and 3 that are two-three-fold higher
than in patients infected with type 1. Interestingly, a significant percentage of HCV genotype 3-infected patients
do not respond to treatment at all. The aim of this paper was to analyse the sequences of fragments of the E2 and
NS5A regions from 33 outpatients infected with genotype 3a, including patients that have responded (SVR) or not
responded (NR) to treatment. HCV RNA was extracted and amplified with specific primers for the NS5A and E2
regions and the PCR products were then sequenced. The sequences obtained covered amino acids (aa) 636-708 in
E2 and in NS5A [including the IFN sensitivity determining region (ISDR), PKR-binding domain and extended V3
region)]. In the E2 and NS5A regions, we did observe aa changes among patients, but these changes were not statistically
significant between the SVR and NR groups. In conclusion, our results suggest that the ISDR domain is not
predictive of treatment success in patients infected with HCV genotype 3a.
Keywords
E2 protein - hepatitis C virus - interferon sensitivity determining region - NS5A protein - PKR-eIF2a phosphorylation homology domain
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