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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 108, No. s1, 2013, pp. 88-91
Bioline Code: oc13192
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 108, No. s1, 2013, pp. 88-91

 en Molecular analysis of an odorant-binding protein gene in two sympatric species of Lutzomyia longipalpis check for this species in other resources s.l.
Dias, Ana Karina Kerche; Bauzer, Luiz Guilherme Soares da Rocha; Dias, Denise Borges dos Santos & Peixoto, Alexandre Afranio

Abstract

Lutzomyia longipalpis check for this species in other resources s.l. is the main vector of American visceral leishmaniasis (AVL) and occurs as a species complex. DNA samples from two Brazilian sympatric species that differ in pheromone and courtship song production were used to analyse molecular polymorphisms in an odorant-binding protein (obp29) gene. OBPs are proteins related to olfaction and are involved in activities fundamental to survival, such as foraging, mating and choice of oviposition site. In this study, the marker obp29 was found to be highly polymorphic in Lu. longipalpis s.l., with no fixed differences observed between the two species. A pairwise fixation index test indicated a moderate level of genetic differentiation between the samples analysed.

Keywords
Lutzomyia longipalpis s.l.; odorant-binding proteins; leishmaniasis

 
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