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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 110, No. 1, 2015, pp. 138-141
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Bioline Code: oc15013
Full paper language: English
Document type: Research Article
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 110, No. 1, 2015, pp. 138-141
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Genetic variability in G2 and F2 region between biological clones of human respiratory syncytial virus with or without host immune selection pressure
Moraes, Claudia Trigo Pedroso; Oliveira, Danielle Bruna Leal; Campos, Angelica Cristine Almeida; Bosso, Patricia Alves; Lima, Hildener Nogueira; Stewien, Klaus Eberhard; Gilio, Alfredo Elias; Vieira, Sandra Elisabete; Botosso, Viviane Fongaro & Durigon, Edison Luiz
Abstract
Human respiratory syncytial virus (HRSV) is an important respiratory pathogens among children between zerofive
years old. Host immunity and viral genetic variability are important factors that can make vaccine production
difficult. In this work, differences between biological clones of HRSV were detected in clinical samples in
the absence and presence of serum collected from children in the convalescent phase of the illness and from their
biological mothers. Viral clones were selected by plaque assay in the absence and presence of serum and nucleotide
sequences of the G2 and F2 genes of HRSV biological clones were compared. One non-synonymous mutation was
found in the F gene (Ile5Asn) in one clone of an HRSV-B sample and one non-synonymous mutation was found in the
G gene (Ser291Pro) in four clones of the same HRSV-B sample. Only one of these clones was obtained after treatment
with the child’s serum. In addition, some synonymous mutations were determined in two clones of the HRSV-A
samples. In conclusion, it is possible that minor sequences could be selected by host antibodies contributing to the
HRSV evolutionary process, hampering the development of an effective vaccine, since we verify the same codon
alteration in absence and presence of human sera in individual clones of BR-85 sample.
Keywords
HRSV; genetic variability; immune selection pressure
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