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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 110, No. 4, 2015, pp. 577-579
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Bioline Code: oc15075
Full paper language: English
Document type: Research Article
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 110, No. 4, 2015, pp. 577-579
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Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections
Arthur Bentes, Gentil; Lanzarini, Natália Maria; Lima, Lyana Rodrigues Pinto; Manso, Pedro Paulo de Abreu; da Silva, Alexandre dos Santos; Junior, Sergio da Silva e Mouta; Guimarães, Juliana Rodrigues; de Moraes, Marcia Terezinha Baroni; Pelajo-Machado, Marcelo & Pinto, Marcelo Alves
Abstract
An increasing amount of research has been conducted on immunoglobulin Y (IgY) because the use of IgY offers
several advantages with respect to diagnostic testing, including its easy accessibility, low cost and translatability to
large-scale production, in addition to the fact that it can be ethically produced. In a previous work, immunoglobulin
was produced and purified from egg yolks (IgY) reactive to hepatitis A virus (HAV) antigens. In the present work,
this anti-HAV-specific IgY was used in an indirect immunofluorescence assay to detect viral antigens in liver biopsies
that were obtained from experimentally infected cynomolgus monkeys. Fields that were positive for HAV antigen
were detected in liver sections using confocal microscopy. In conclusion, egg yolks from immunised hens may be a
reliable source for antibody production, which can be employed for immunological studies.
Keywords
immunoglobulin Y; hepatitis A virus; indirect immunofluorescence
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