This study aimed to verify the diversity of Culicidae species and their frequency of infection with flaviviruses and
alphaviruses in Cuiabá, state of Mato Grosso, Brazil. Mosquitoes were captured with Nasci aspirators and hand net
in 200 census tracts, identified alive at species level and pooled in one-20 (11,090 mosquitoes, 14 species). Female
pools (n = 610) were subjected to multiplex seminested-reverse transcription-polymerase chain reaction (RT-PCR)
for 11 flavivirus and five alphavirus. Positive pools were tested by single RT-PCR followed by nucleotide sequencing,
by RT-PCR for
E1 gene [Mayaro virus (MAYV)] and by inoculation in Vero cells (MAYV) or C6/36 cells (flaviviruses).
One/171
Aedes aegypti
was positive for dengue virus (DENV)-1, 12/403
Culex quinquefasciatus
, and four/171
Ae. aegypti for MAYV, which was isolated from two pools containing two nonengorged females of
Ae. aegypti and
two of
Cx. quinquefasciatus. DENV-4 was detected in 58/171 pools of
Ae. aegytpi, 105/403
Cx. quinquefasciatus,
two/five
Psorophora
sp., two/11
Psorophora varipes
/
Psorophora albigenu
, one/one
Sabethes chloropterus
, two/five
Culex bidens
/
Culex interfor
, and one/one
Aedes
sp. DENV-4 was isolated from two pools containing three and 16
nonengorged
Cx. quinquefasciatus females. Phylogenetic analysis revealed MAYV belongs to genotype L, clustering
with human samples of the virus previously identified in the city. Cuiabá has biodiversity and ecosystem favourable
for vector proliferation, representing a risk for arbovirus outbreaks.
