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Complete genome of a dengue virus serotype 4 strain from Amazonas, Brazil
Nascimento, Valdinete Alves do; Souza, Victor Costa de & Naveca, Felipe Gomes
Abstract
Dengue virus (DENV) infections represent a significant concern for public health worldwide, being considered
as the most prevalent arthropod-borne virus regarding the number of reported cases. In this study, we report the
complete genome sequencing of a DENV serotype 4 isolate, genotype II, obtained in the city of Manaus, directly
from the serum sample, applying Ion Torrent sequencing technology. The use of a massive sequencing technology
allowed the detection of two variable sites, one in the coding region for the viral envelope protein and the other in
the nonstructural 1 coding region within viral populations.
Keywords
Brazilian Amazon; dengue virus; DENV-4; viral genome; next-generation sequencing
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