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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 111, No. 7, 2016, pp. 423-432
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Bioline Code: oc16064
Full paper language: English
Document type: Research Article
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 111, No. 7, 2016, pp. 423-432
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Phlebotomine sandfly (Diptera: Psychodidae) diversity and their Leishmania DNA in a hot spot of American Cutaneous Leishmaniasis human cases along the Brazilian border with Peru and Bolivia
Teles, Carolina Bioni Garcia; dos Santos, Ana Paula de Azevedo; Freitas, Rui Alves; de Oliveira, Arley Faria José; Ogawa, Guilherme Maerschner; Rodrigues, Moreno Souza; Pessoa, Felipe Arley Costa; Medeiros, Jansen Fernandes & Camargo, Luís Marcelo Aranha
Abstract
In this study, we identified the phlebotomine sandfly vectors involved in the transmission of American Cutaneous
Leishmaniasis (ACL) in Assis Brasil, Acre, Brazil, which is located on the Brazil-Peru-Bolivia frontier. The genotyping
of Leishmania in phlebotomines was performed using polymerase chain reaction (PCR) and PCR-restriction
fragment length polymorphism. A total of 6,850 sandflies comprising 67 species were captured by using CDC light
traps in rural areas of the municipality. Three sandfly species were found in the state of Acre for the first time: Lutzomyia
georgii, Lu. complexa and Lu. evangelistai. The predominant species was Lu. auraensis/Lu. ruifreitasi and
Lu. davisi (total 59.27%). 32 of 368 pools were positive for the presence of Leishmania DNA (16 pools corresponding
to Lu. davisi, and 16 corresponding to Lu. auraensis/Lu. ruifreitasi), with a minimal infection prevalence of 1.85% in
Lu. davisi and 2.05% in Lu. auraensis/Lu. ruifreitasi. The Leishmania species found showed maximum identity with
L. (Viannia) guyanensis and L. (V.) braziliensis in both phlebotomine species. Based on these results and similar
scenarios previously described along the Brazil/Peru/Bolivia tri-border, the studied area must take into consideration
the possibility of Lu. davisi and Lu. auraensis/Lu. ruifreitasi as probable vectors of ACL in this municipality.
Keywords
sandflies; Leishmania; disease vectors; Assis Brasil Municipality; Acre; Brazil
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