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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 111, No. 9, 2016, pp. 570-576
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Bioline Code: oc16088
Full paper language: English
Document type: Research Article
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 111, No. 9, 2016, pp. 570-576
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Detection of Plasmodium in faeces of the New World primate Alouatta clamitans
Assis, Gabriela Maíra Pereira de; Alvarenga, Denise Anete Madureira de; Costa, Daniela Camargos; Souza Junior, Júlio César de; Hirano, Zelinda Maria Braga; Kano, Flora Satiko; Sousa, Taís Nóbrega de & Brito, Cristiana Ferreira Alves de
Abstract
Plasmodium falciparum and Plasmodium vivax have evolved with host switches between non-human primates
(NHPs) and humans. Studies on the infection dynamics of Plasmodium species in NHPs will improve our understanding
of the evolution of these parasites; however, such studies are hampered by the difficulty of handling animals in the
field. The aim of this study was to detect genomic DNA of Plasmodium species from the faeces of New World monkeys.
Faecal samples from 23 Alouatta clamitans from the Centre for Biological Research of Indaial (Santa Catarina,
Brazil) were collected. Extracted DNA from faecal samples was used for molecular diagnosis of malaria by nested
polymerase chain reaction. One natural infection with Plasmodium simium was identified by amplification of DNA
extracted from the faeces of A. clamitans. Extracted DNA from a captive NHP was also used for parasite genotyping.
The detection limit of the technique was evaluated in vitro using an artificial mixture of cultured P. falciparum in NHP
faeces and determined to be 6.5 parasites/µL. Faecal samples of New World primates can be used to detect malaria
infections in field surveys and also to monitor the genetic variability of parasites and dynamics of infection.
Keywords
New World primates; Alouatta clamitans; simian malaria; Plasmodium simium; Plasmodium vivax; non-invasive sampling
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