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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 111, No. 11, 2016, pp. 663-669
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Bioline Code: oc16106
Full paper language: English
Document type: Research Article
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 111, No. 11, 2016, pp. 663-669
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Influence of IL-6, IL-8, and TGF-β1 gene polymorphisms on the risk of human papillomavirus-infection in women from Pernambuco, Brazil
Lima Júnior, Sérgio Ferreira de; Tavares, Mayara Mansur Fernandes; Macedo, Jamilly Lopes de; Oliveira, Renata Santos de; Heráclio, Sandra de Andrade; Maia, Maria de Mascena Diniz; Souza, Paulo Roberto Eleutério de; Moura, Ronald & Crovella, Sergio
Abstract
Human papillomavirus (HPV) infections are strongly associated with the development of cervical intraepithelial
neoplasias and invasive cervical cancer. Polymorphisms in cytokine-encoding genes and behavioural cofactors
could play an important role in protecting an individual against viral infections and cancer. Here, we investigated
whether IL-6 -174 G>C, IL-8 +396 G>T, and TGF-β1 +869 G>C and +915 G>C polymorphisms were associated
with susceptibility to HPV infection in women from north-east (Pernambuco) Brazil. We analysed 108 healthy uninfected
women (HC) and 108 HPV-positive women with cervical lesions. Genetic polymorphisms were assessed using
Sanger sequencing and polymerase chain reaction-restriction fragment length polymorphism. Comparison of the
distribution of the genotypic and allelic frequencies of the IL-18 +396 T>G polymorphism between HPV infected
woman an uninfected controls showed that the GG genotype and G allele were both more frequent in the HC group,
and were associated with protection from HPV infection (p = 0.0015; OR = 0.29 CI95% = 0.13-0.61; p = 0.0005; OR
= 0.45 CI95% 0.29-0.7, respectively). Individuals from the control group could have previously had HPV infection
that was spontaneously eliminated; however, it was undetectable at the time of sample collection. Based on our findings,
we hypothesize that the IL-8 +396 G>T polymorphism could interfere with susceptibility to HPV infection, by
modulating the ability of immune system to fight the virus.
Keywords
polymorphisms; cytokine; host genome; HPV
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