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Correlation between the BACTEC MGIT 960 culture system with Genotype MTBDRplus and TB-SPRINT in multidrug resistant Mycobacterium tuberculosis clinical isolates from Brazil
Dantas, Nayanne Gama Teixeira; Suffys, Philip Noel; Carvalho, Wânia da Silva; Gomes, Harrison Magdinier; Almeida, Isabela Neves de; Figueiredo, Lida Jouca de Assis; Gonçalves, Alan Douglas; Gomgnimbou, Michel Kireopori; Refregier, Guislaine; Sola, Christophe & Miranda, Silvana Spíndola de
Abstract
BACKGROUND The accurate detection of multidrug-resistant tuberculosis (MDR-TB) is critical for the application of appropriate
patient treatment and prevention of transmission of drug-resistant Mycobacterium tuberculosis isolates. The goal of this study was
to evaluate the correlation between phenotypic and molecular techniques for drug-resistant tuberculosis diagnostics. Molecular
techniques used were the line probe assay genotype MTBDRplus and the recently described tuberculosis-spoligo-rifampin-isoniazid
typing (TB-SPRINT) bead-based assay. Conventional drug susceptibility testing (DST) was done on a BACTECTM MGIT 960 TB.
METHOD We studied 80 M. tuberculosis complex (MTC) clinical isolates from Minas Gerais state, of which conventional DST
had classified 60 isolates as MDR and 20 as drug susceptible.
FINDINGS Among the 60 MDR-TB isolates with MGIT as a reference, sensitivity, specificity, accuracy, and kappa for rifampicin
(RIF) resistance using TB-SPRINT and MTBDRplus, were 96.7% versus 93.3%, 100.0% versus 100.0%, 97.5% versus 95.0%
and 0.94 versus 0.88, respectively. Similarly, the sensitivity, specificity, accuracy, and kappa for isoniazid (INH) resistance were
85.0% and 83.3%, 100.0% and 100.0%, 88.8% and 87.5% and 0.74 and 0.71 for both tests, respectively. Finally, the sensitivity,
specificity, accuracy, and kappa for MDR-TB were 85.0% and 83.3%, 100.0% and 100.0%, 88.8% and 87.5% and 0.74 and 0.71
for both tests, respectively.
MAIN CONCLUSIONS Both methods exhibited a good correlation with the conventional DST. We suggest estimating the cost-effectiveness
of MTBDRplus and TB-SPRINT in Brazil.
Keywords
tuberculosis; multidrug-resistant; genotype; molecular diagnosis
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