|
Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 94, No. 3, 1999, pp. 305-309
|
Bioline Code: oc99058
Full paper language: English
Document type: Research Article
Document available free of charge
|
|
Memórias do Instituto Oswaldo Cruz, Vol. 94, No. 3, 1999, pp. 305-309
en |
Use of Monoclonal Antibodies for the Identification of Leishmania spp. Isolated from Humans and Wild Rodents in the State of Campeche, Mexico
Silvia B CantoLara; Nicole R Van Wynsberghe; Alberto Vargas-González; Fanny F Ojeda-Farfán & Fernando J Andrade-Narváez
Abstract
The genus Leishmania includes 30 described species which infect a wide variety of mammalian hosts. The precise identification of leishmanial parasites at the species level is very important in order to determine whether an organism, causing the disease in a given area, is of the same biotype as that found
in suspected mammalian reservoirs. The objectives of the present study were (1) to identify leishmanial parasites isolated from humans and wild rodents from the State of Campeche, an endemic focus of localized cutaneous leishmaniasis (LCL) in southern Mexico, using an indirect immunofluorescent assay (IFA) with
monoclonal antibodies (Mabs); and (2) to determine if the parasites of the two types of hosts were of the same biotype. All the wild rodents (six Ototylomys phyllotis, eight Oryzomys melanotis, five Peromyscus yucatanicus and two Sigmodon hispidus) and 96% (24/25) of the human isolates
were identified as Leishmania (L.) mexicana confirming that this specific LCL focus is a wild zoonosis. The presence of one human isolate of {B}L. {/B}(Viannia) braziliensis in the State of Campeche, confirmed the importance of an accurate taxonomic identification at species level.
Keywords
Leishmania (Leishmania) mexicana - Leishmania (Viannia) braziliensis - monoclonal antibodies - human - rodents - Mexico
|
|
© Copyright 1999 Fundacao Oswaldo Cruz - Fiocruz Alternative site location: http://memorias.ioc.fiocruz.br
|
|