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Iranian Journal of Pediatrics
Tehran University of Medical Sciences Press
ISSN: 1018-4406
EISSN: 2008-2150
Vol. 26, No. 6, 2016, pp. 1-8
Bioline Code: pe16098
Full paper language: English
Document type: Research Article
Document available free of charge

Iranian Journal of Pediatrics, Vol. 26, No. 6, 2016, pp. 1-8

 en Comparative Analysis of Fecal Microbiota in 5 - 8-Year-old Children with and without Cow Milk Protein Allergy
Guo, Ling; Bai, Hongjian; Dong, Yanru; Huang, Dong Xu; Zhang, Xinjie; Gong, Shaoying; Zhao, Zuanying & Fei, Peng

Abstract

Background: The bacterial diversity and microbial communities in the feces of children with cow milk protein allergy (CMPA) may be altered; these changes have not been clearly elucidated in 5 - 8-year-old children with CMPA.
Objectives: The aim of this study was to compare the bacterial diversity and microbial communities of feces in 5 - 8-year-old children with CMPA and those of healthy children of the same age.
Methods: Stool samples from 12 children with CMPA and 12 healthy individuals were collected. Total bacterial DNA of all samples were extracted and analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) using bacterial v3 universal primers and the species-specific primers of Bifidobacterium check for this species in other resources , Lactobacillus check for this species in other resources , and Clostridium coccoides check for this species in other resources . The preponderant bacterial bands were identified by gene sequencing.
Results: The results revealed that the diversity of fecal dominant microbiota and C. coccoides group in children with CMPA were significantly higher (P < 0.05) than in healthy children. Meanwhile, a lower degree of diversity in the Bifidobacterium group and no difference in the diversity of the Lactobacillus group were found in the feces of children with CMPA. Bacteroides check for this species in other resources , Clostridium, and Escherichia coli check for this species in other resources were more abundant in the fecal microbiota of CMPA children than in healthy controls. In addition, C. celerecrescens check for this species in other resources was found only in the fecal microbiota of CMPA children, whereas B. bifidum check for this species in other resources was found only in the feces of healthy children.
Conclusions: There were some notable changes in the fecal microbiota of 5 - 8-year-old children with CMPA.

Keywords
Cow Milk Protein Allergy; Fecal Microbiota; Children

 
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