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Indian Journal of Pharmacology
Medknow Publications on behalf of Indian Pharmacological Society
ISSN: 0253-7613 EISSN: 0253-7613
Vol. 36, No. 3, 2004, pp. 167-170
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Bioline Code: ph04056
Full paper language: English
Document type: Research Article
Document available free of charge
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Indian Journal of Pharmacology, Vol. 36, No. 3, 2004, pp. 167-170
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A semi-quantitative method for the estimation of adenosine A1 receptor mRNA levels in rat kidney
Döndas N
Abstract
OBJECTIVE:
To develop Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) method for the estimation of adenosine A1 receptor mRNA levels in rat kidney.
MATERIAL AND METHODS:
Total cellular RNA was isolated from whole rat kidney by small-scale total RNA preparation protocol and it was reverse transcribed into cDNA. The cDNA was subjected to PCR amplification using gen specific primers. The amplified cDNA was evaluated by gel electrophoresis and the intensity of the bands were visualized and quantitated with a FujiBAS 1000 PhosphorImager. Then the adenosine A1 receptor mRNA levels were extrapolated from the standard curve.
RESULTS:
Adenosine A1 receptor mRNA levels in rat kidney were measured as: 1.30 ± 0.17 X 107 copies of adenosine A1 receptor transcript/mg total RNA.
CONCLUSION:
The RT-PCR method developed for the estimation of adenosine A1 receptor mRNA levels in rat kidney is sensitive and reliable.
Keywords
RT-PCR, cellular RNA, cDNA
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© Copyright 2004 Indian Journal of Pharmacology. Alternative site location: http://www.ijp-online.com
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