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Anti-dyslipidemic and Antioxidant Potentials of Methanol Extract of Kalanchoe crenata Whole Plant in Streptozotocin-induced Diabetic Nephropathy in Rats
Fondjo, Foyet Angèle; Kamgang, René; Oyono, Jean-Louis Essame & Yonkeu, Jeanne Ngongang
Abstract
Purpose:
The activity of the methanol extract of the whole plant of Kalanchoe crenata (MEKC) was
studied for the treatment of diabetes-induced nephropathy in rats.
Methods:
Five-day old Wistar rats received a single intraperitoneal streptozotocin injection (90 μg/kg
body weight) to induce diabetes. Kidney disease onset in the rats was observed six weeks after
diabetes induction. The rats were orally administered MEKC (0, 50 and 68 mg/kg) or glibenclamide (5
mg/kg), once daily for 6 weeks. Blood and urine glucose, proteins, lipids, creatinine, malondialdehyde
(MDA), superoxide dismutase (SOD) and catalase (CAT) were then evaluated.
Results:
After 6 weeks of treatment, 50 and 68 mg/kg MEKC, and glibenclamide significantly (p < 0.01)
decreased glycaemia (-35, -44 and -39 %), glycosuria (-38, -47 and -61 %) and proteinuria (-82, -80 and
-72 %) in diabetes-nephropathic rats. The extract (68 mg/kg) decreased MDA by up to -44 % (blood), -
35 % (liver) and -34 % (kidney); increased SOD up to 257 % (blood), 116 % (liver) and 118 % (kidney);
and CAT by up to 176 % (blood), 78 % (liver) and 96 % (kidney) in the rats, compared with nephropathic
control. The extract (50 and 68 mg/kg, respectively) lowered (p < 0.01) total cholesterolemia (-24 and -
27 %), blood triglycerides (-55 and -54 %), blood LDL cholesterol (-48 and -59 %), but increased blood
HDL cholesterol (71 and 58 %). Overall, atherogenic index was decreased by 31 %.
Conclusion:
The results indicate that MEKC holds promise for the development of a standardized
phytomedicine for diabetes mellitus and kidney disease treatment.
Keywords
Diabetes, Dyslipidemia, Antioxidant, Kalanchoe crenata extract, Nephropathy.
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