Purpose: To investigate the effect of total glucosides of
Paeonia lactiflora
(TGPL) on nitric oxide (NO)
production and its potential mechanism in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage
cells.
Methods: RAW264.7 cells were treated with 10 - 300 μg/ml TGPL and 1 μg/ml LPS. Cell survival was
determined by MTT assay. NO level was determined by Griess reaction assay. Inducible NO synthase
(iNOS) expression and inhibitor-κBα (IκBα) degradation were determined by Western blot assay. DNA
binding activity of NF-κB was determined by ELISA assay using Trans AM™ kit for p65.
Results: The concentrations of TGPL (10 - 300 μg/ml) used in this study did not affect cell survival of
RAW264.7 cells, which suggest that 10-300 μg/ml TGPL did not show cytotoxic effect on RAW264.7
cells. NO level and iNOS protein expression significantly increased in LPS-stimulated RAW264.7 cells
compared to the unstimulated cells. However, 10 - 300 μg/ml TGPL significantly decreased LPS-induced
NO level and iNOS protein expression compared to LPS-stimulated RAW264.7 cells alone.
Furthermore, 10 - 300 μg/mL TGPL significantly reduced the content of IκBα protein in LPS-stimulated
RAW264.7 cells, which suggests that TGPL inhibited LPS-induced degradation of IκBα protein. TGPL
remarkably repressed LPS-induced DNA binding activity of P65 in RAW264.7 cells.
Conclusion: These findings suggest that TGP inhibits NO production and iNOS expression through
suppression of NF-κB activation in LPS-stimulated RAW264.7 cells.
