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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-5996
Vol. 13, No. 8, 2014, pp. 1347-1352
Bioline Code: pr14185
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 13, No. 8, 2014, pp. 1347-1352

 en Fatty Acid Profile and Physicochemical Properties of Landolphia owariensis check for this species in other resources P. Beauv Stringy Seed Oil
Okonkwo, Tochukwu J.N.; Obonga, Wilfred O. & Okonkwo, Chinedu J.O.

Abstract

Purpose: To elucidate lipid profile of Landolphia owariensis check for this species in other resources P. Beauv stringy seed oil, the bulk physicochemical properties of its neutral lipid (NL), and also to ascertain its nutritional properties, safety and industrial applications.
Methods: The neutral lipid was recovered by macerating 500 g of L. owariensis stringy seed pulp in 1 L of petroleum ether (40 – 60 ºC boiling range) for 48 h. Methyl esters of the inherent fatty acids were generated by transmethylation while the physicochemical properties of the NL was determined by official methods of the Association of Official Analytical Chemists (AOAC).
Results: The acid, iodine, saponification and peroxide values were 2.81 ± 0.01 mg KOH/g, 67.26 ± 1.05 mg I/100 g, 219.38 ± 2.69 mg KOH/g and 6.00 ± 0.28 mEq/kg, respectively. Thiobarbituric acid reactive substance (TBARS) value (6.10 ± 0.24 mg MDA/kg) of the NL further confirmed the stability of NL (neutral lipid) against oxidative rancidity. Gas chromatographic analysis of the methyl ester mix of L. owariensis stringy seed pulp NL indicate hexadecanoic acid (palmitic acid) as the principal fatty acid with an absolute proportion of 54.38 %. Palmitoleic acid (18.54 %) and linoleic acid (9.56 %) ranked second and third, respectively, while the saturated/unsaturated fatty acid ratio was 2.53.
Conclusion: The neutral lipid of seed oil exhibited strong stability to ketonic and oxidative rancidity, and is safe for edible purposes. Its relatively high unsaturated fatty acid proportion (28.29 %) indicates that L. owariensis stringy seed pulp fixed oil possesses good nutritional qualities.

Keywords
Landolphia owariensis; Stringy seed pulp; Lipid profile; Fatty acids; Palmitic acid; Palmitoleic acid; Linoleic acid

 
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