Purpose: To evaluate the ultraviolet A (UVA) protection and anti-inflammatory activity of ganoderol A
extracted from
Ganadermalucidum
.
Methods: The cytotoxicity and
in vitro protective effect of ganoderol A against UVA damage were
evaluated by MTT assay. Apoptosis and cell-cycle arrest of NIH/3T3 fibroblast cells were assayed by
fluorescence-activated cell sorting (FCS). Expression of monocyte chemotactic protein-1 (MCP-1) and
inducible nitric oxide synthase (iNOS) were determined using quantitative real-time polymerase chain
reaction (qPCR).
Results: The results indicate that the maximal non-toxic concentration of ganoderol A in NIH/3T3 cells
and RAW 264.7 macrophages was 50 and 25 μg/mL respectively. DNA in the tails and tail length
decreased by 55 and 70 %, respectively, in the group pretreated with ganoderol A compared with the
UVA-treated group. G1 phase cells decreased by 23 %, whereas the number of apoptotic cells returned
to normal. The expression of MCP-1 and iNOS declined to 60 and 15 %, respectively, compared with
LPS-stimulated group.
Conclusion: Ganoderol A has significant anti-inflammatory activity and protection against UVA
damage, thus suggesting that the compound is a candidate for the development of a suitable product to
protect skin from UV-induced photoaging.
