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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996 EISSN: 1596-5996
Vol. 14, No. 5, 2015, pp. 815-821
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Bioline Code: pr15107
Full paper language: English
Document type: Research Article
Document available free of charge
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Tropical Journal of Pharmaceutical Research, Vol. 14, No. 5, 2015, pp. 815-821
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Study on the Influence of Sulfur Fumigation on Chemical Constituents of Angelicae dahurica Radix (Baizhi)
Jiang, Yun-Bin; Lu, Xiao-Lin; Peng, Wei; Deng, Wei & Ma, Yu-Ying
Abstract
Purpose: To study the influence of different sulfur fumigation time and dosage on the chemical
constituents of Baizhi Angelicae dahurica Radix).
Methods: The relationship of chemical constituents in Baizhi with different sulfur fumigation time and
dosages was evaluated by high performance liquid chromatography (HPLC) fingerprint and
chemometrics methods, including similarity analysis (SA), hierarchical clustering analysis (HCA) and
principal component analysis (PCA). HPLC was carried out on a Diamonil C18 column; linear gradient
elution was performed with acetonitrile and water; column temperature, injection volume of sample, flow
rate, detection wavelength and testing time were 30 °C, 20 μL, 1 mL/min, 310 nm and 75 min
respectively.
Results: The chemical constituents of Baizhi significantly (p < 0.05) decreased after sulfur fumigation
though sulfur fumigation time and dosage were at low levels – 2 h and 25 g (sulphur)/10 kg (Baizhi),
respectively.
Conclusion: Sulfur fumigation is not a desirable method for field processing of Baizhi even when sulfur
fumigation time and dosage were short and small.
Keywords
Baizhi; Angelicae dahurica; Sulfur fumigation; HPLC-DAD; Fingerprint; Chemometrics methods
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