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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996 EISSN: 1596-5996
Vol. 14, No. 11, 2015, pp. 2077-2082
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Bioline Code: pr15272
Full paper language: English
Document type: Research Article
Document available free of charge
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Tropical Journal of Pharmaceutical Research, Vol. 14, No. 11, 2015, pp. 2077-2082
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Solid-Phase Extraction Combined with High Performance Liquid Chromatography-Diode Array Detector for Rapid Determination of Estrogens in Milk
Yan, Qinghua; Yang, Li & Li, Sufang
Abstract
Purpose: To develop a simple but effective method for the quantitative analysis of estrogens in milk.
Methods: Solid-phase extraction method was employed for the extraction of the estrogen from milk and
high performance liquid chromatography-diode array detector (HPLC-DAD) was used for the
determination of estrogen.
Results: Optimal chromatographic conditions were achieved on an Eclipse XDB-C18 column at a flow
rate of 0.8 mL/min at room temperature. The products were monitored using a DAD detector set at 280
nm. The retention time of the three main estrogens were 15 min with a gradient program of
acetonitrile/water, from 0 to 3 min, holding 35 % acetonitrile; from 4 to 7 min, ramped to 55 %
acetonitrile; from 8 to 20 min, maintained at 55 % acetonitrile. The results showed that the calibration
curve had good linearity within the concentration range of 0.5 - 8.0 μg/mL with correlation coefficient
(R2) in the range of 0.9929-0.9936. The limit of detection was in the range of 0.025 - 0.045 μg/mL while
mean recovery of estrogen from milk samples varied from 92.6 to 104.1 %. Satisfactory precision was
obtained both for intra-assay (RSD, 1.8 to 4.4 %) and inter-assay (RSD, 2.0 to 4.7 %).
Conclusion: The proposed method is environmentally friendly, inexpensive and convenient, and should
be helpful in analyzing estrogens in biological, environmental and food samples.
Keywords
Solid-phase extraction; Milk; Estrogens; High performance liquid chromatography-diode array detector
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