Purpose: To evaluate the impact of three different extraction methods on yield, physicochemical
properties and bioactive ingredients of Raphanus sativus seed oil.
Methods: Raphanus Sativus
seed oil was prepared by traditional solvent extraction (SE), super-critical
carbon dioxide extraction (SCE) and sub-critical propane extraction (SPE). The yield, physicochemical
properties, fatty acid composition and oxidative stability of the oil extracts were compared. The contents
of tocopherol and sulforaphene in the oils were also determined.
Results: The oil yield obtained by SPE, SE and SCE were 33.69, 27.17 and 24.10 %, respectively.
There were no significant differences in physicochemical properties and fatty acid compositions of oils
extracted by the three methods. However, SCE oil had the best oxidative stability, and highest contents
of vitamin E and sulforaphene, followed by oils from SPE and SE.
Conclusion: SCE is highly selective for tocopherol and sulforaphene, which could explain its high oil
oxidative stability. These results suggest that of the three extraction methods, SCE is best suited for
preparing medicinal radish seed oil.
