Purpose: To investigate the crude oil yield of eight different varieties of soybean (Glycine max L.) seeds
after gamma radiation, and also to evaluate the antimicrobial activity and the chemical composition of
G.max-Ataem7.
Methods: The seeds were irradiated with doses of 0 (control), 100, 200, 300, 400 and 500 Gy gamma
radiation. Irradiation was performed in a cesium (Ce137) source. Extraction of the seeds was done with
Soxhlet apparatus using petroleum ether. Antimicrobial activity was determined by the standard disc
diffusion method. The chemical composition of the extracts was elucidated using gas chromatographymass
spectrometry (GS-MS)
Results: The highest crude oil yield was obtained at 300 Gy and and content of 35.09 % in Ataem7
seeds. There was a decrease in the total content of chlorophyll in Mutant1 (M1) plants after gamma
radiation. However, the level of carotenoid increased in M1 plants. Extracts of the G.max-Ataem7
demonstrated strong antibacterial activity against
S. aureus
ATCC 25923 and
E.coli
ATCC 25922. The
major components of G.max-Ataem7 were linoliec acid (C18:2n6) and oleic acid (C18:1n9) with a
content of 60.31 and 21.64 %, respectively.
Conclusion: The results show that irradiation of is also can be improved if treated with appropriate
doses of irradiation.
